Primer

Part:BBa_K4816005:Design

Designed by: Julia Alexander   Group: iGEM23_Bonn-Rheinbach   (2023-09-25)


norB primer forward


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Specific binding to norB gene. Containing of a restriction site for restriction digest and cloning into plasmid vector after PCR amplification.


Source

Sequence was designed on the basis of the DNA sequence for the norB gene of Paracoccus denitrificans found on KEGG https://www.genome.jp/entry/pde:Pden_2483

References